PLX058470

GSE94155: A next generation sequencing based approach to identify extracellular vesicle mediated mRNA transfers between cells

• Organsim human
• Type RNASEQ
• Target gene
• Project ARCHS4

Purpose: Exosomes and other extracellular vesicles (EVs) have emerged as an important mechanism of cell-to-cell communication. In this work, we present a novel next generation of sequencing (NGS) based approach to identify EV mediated mRNA exchanges between co-cultured adipocyte and macrophage cells.; Methods:We performed molecular and genomic profiling and jointly considered data from RNA sequencing (RNA-seq) and genotyping to track the sequence varying mRNAs transferred between cells.; Results: We identified 8 mRNAs being transferred from macrophages to adipocytes and 21 mRNAs being transferred in the opposite direction. These mRNAs represented biological functions including extracellular matrix, cell adhesion, glycoprotein, and signal peptides.; Conclusions: Our study sheds new light on EV mediated RNA communications between adipocyte and macrophage cells, which may play a significant role in developing insulin resistance in diabetic patients. This work establishes a new method that is widely applicable to examining genetic material exchanges in many other cellular systems. SOURCE: Zhidong Tu (zhidong.tu@mssm.edu) - TU Icahn School of Medicine at Mount Sinai