PLX137663

GSE88953: Impact of hypothermic preservation of mouse kidney resident immune cells

• Organsim mouse
• Type RNASEQ
• Target gene
• Project ARCHS4

With the growing interest in studying primary tissue samples by single cell transcriptome analysis, there is an emerging demand for a preservation strategy that enables sample transportation and storage. In this study, we describe a simple and general strategy that preserves primary tissues at hypothermic temperature. Using FACS and single-cell RNAseq, we demonstrated the effectiveness of this strategy in maintaining cell viability, cell population heterogeneity, and cell transcriptome integrity for primary tissues that underwent up to 3 days of preservation. SOURCE: Wanxin Wang (wanxinw@stanford.edu) - Wanxin Wang Stanford Univeristy