PLX271515
GSE84582: Global gene expression profiles of cardiac progenitors differentiated from human pluripotent stem cells in 3D culture under simulated microgravity
- Organsim human
- Type RNASEQ
- Target gene
- Project ARCHS4
Methods: RNA-seq libraries were prepared using the Illumina TruSeq RNA kit and the TrueSeq method was employed for mRNA enrichment. The libraries were quantified and samples were multiplexed in each lane of the flowcell. Cluster generation was performed and then sequenced on the Illumina HiSeq1000 system. Reads were mapped on the Human Genome Reference and normalized expression table was generated.; Results: Among differentially expressed genes, 53 of them were up-regulated and 75 were down-regulated.; Conclusions: Data demonstrate increased expression of genes associated with growth, development, and pro-survival in cardiac progenitors cultured under simulated microgravity compared with those cultured under standard gravity. SOURCE: Rajneesh Jha (rajneesh.jha@emory.edu) - Stem Cell Cardiomyocytes Lab Emory University School of Medicine
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