PLX143143

GSE72764: RNAseq analysis of the duodenum of intestine-specific adult TN mutant mice

• Organsim mouse
• Type RNASEQ
• Target gene
• Project ARCHS4

Aim: Transcriptional analysis of the duodenum of adult Nkx2.2flox/TN;Villin-Cre (TNint) mice versus control; Methods: 2 cm of the duodenum (as measured from the stomach) of 6 week old control and mutant mice were dissected and total RNA extracted. Libraries were prepared from total RNA (RIN>8) with the TruSeq RNA prep kit (Illumina) and sequenced using the HiSeq2000 (Illumina) instrument. More than 20 million reads were mapped to the mouse genome (UCSC/mm9) using Tophat (version 2.0.4) with 4 mismatches and 10 maximum multiple hits. Significantly differentially expressed genes were calculated using DEseq.; Results: 299 genes with a p-value <0.05 were significantly changed. Among these are some enteroendocrine hormones.; Conclusion: The TN domain of Nkx2.2 regulates specification of some enteroendocrine cells SOURCE: Lori Sussel (lgs2@columbia.edu) - Columbia University