PLX191178

GSE58453: Next Generation Sequencing of Wild-Type FVB/NJ Mouse Primary Cardiomyocyte and Cardiac Fibroblast Polyadenylated RNA and small RNA

  • Organsim mouse
  • Type RNASEQ
  • Target gene
  • Project ARCHS4

Cells from three adult, wild-type, FVB hearts were separated into cardiomyocyte and nonmyocyte fractions using Langendorff perfusion, collagenase digestion and gravity filtration; total RNA was prepared immediately from myocytes, while nonmyocytes were passaged twice to yield a culture of largely fibroblasts from which total RNA was prepared. SOURCE: Scot,J,Matkovich (smatkovich@gmail.com) - Washington University School of Medicine

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