PLX103997

GSE58318: Next Generation Sequencing and Quantitative Analysis of Wild Type and p110gamma-/- macrophages

• Organsim mouse
• Type RNASEQ
• Target gene
• Project ARCHS4

Purpose: The goals of this study were to identify quantitative gene expression differences between macrophages derived from wild type and PI3Kgamma null macrophages; Methods: mRNA profiles of MCSF, IL4 and IFNg/LPS stimulated macrophage wild-type (WT) and PI3Kinase gamma knockout (p110g-/-) mice were generated by single read deep sequencing, in triplicate, using Illumina HiSeq2000. The sequence reads that passed quality filters were aligned to mouse transcriptome using the bowtie2 aligner. Gene-level summaries were normalized and analyzed for differential expression using DESeq. qRTPCR validation was performed using SYBR Green assays.; Conclusions: Our study represents the first detailed analysis of the role of p110g in the control of the macrophage immune response, with biological replicates, generated by RNA-seq technology. SOURCE: Roman Sasik (rsasik@ucsd.edu) - UCSD