PLX065927

GSE47114: RNA-Guided Human Gene Activation by Cas9/CRISPR-Based Engineered Transcription Factors

• Organsim human
• Type RNASEQ
• Target gene
• Project ARCHS4

Synthetic transcription factors can be applied in many areas of biotechnology, medicine, and basic research. In contrast to current methods based on engineering new DNA-binding proteins, we show that Cas9 fused to a transcriptional activation domain can be targeted by combinations of guide RNA molecules to induce the expression of endogenous human genes.This simple approach for targeted gene activation circumvents the need for engineering new proteins and will enable widespread synthetic gene regulation. SOURCE: Timothy,E,Reddy (tim.reddy@duke.edu) - ReddyLab Duke University