PLX033761

GSE42420: CFIm25 links alternative polyadenylation to glioblastoma tumour suppression

• Organsim human
• Type RNASEQ
• Target gene
• Project ARCHS4

Purpose: To identify all of the APA targets of CFIm25 on a global scale and develop an algorithm that can idenitify APA events from standard RNA-seq data; ; Methods: RNA from HeLa cells treated with control siRNA and CFIm25 siRNA were subject to RNA-Seq. Using a custom-designed algorithm to mine RNA-seq data for novel APA events regulated by CFIm25.; ; Results: We identified over 1,400 genes with shortened 3UTRs after CFIm25 knockdown. Importantly, we show that as a consequence of APA, many of these mRNAs have greatly enhanced protein expression due to the loss of destabilizing features within the 3UTR.; ; Conclusions: Our study underscored the critical function of the CFIm complex members in governing APA and establish a previously unknown link between APA and metabolic pathways important for tumor progression. SOURCE: Zheng Xia (zxia@bcm.edu) - Li Lab Baylor College of Medicine