PLX152973

GSE154567: Single cell RNAseq reveal covid19 patient immune response

• Organsim human
• Type RNASEQ
• Target gene
• Project ARCHS4

Eighteen patients admitted to CSMC and diagnosed with COVID19 by RT-PCR were stratified into COVID19 mild/moderate, severe, and recovery groups (n=5-6/group). Venous blood was collected into EDTA coated tubes and centrifuged to separate plasma and buffy coat. Plasma was collected and frozen at -80C, and the buffy coat was collected into cryo-preservation media and frozen at -80C. Recovered cells are sorted for live-dead staining, then labeled with Totalseq A hashing antibodies and fixed with methanol. Fixed single cells were further captured using 10x chromium Next GEM 3 prime v3.1 kit SOURCE: Changfu Yao (changfu.yao@cshs.org) - Stripp Lab cedars-sinai medical center