PLX074857

GSE154069: The Role of Satb1 in Shaping the Repertoire of T Cell Receptor Genes

  • Organsim mouse
  • Type RNASEQ
  • Target gene
  • Project ARCHS4

To explore the role of Satb1 in rearrangement and repertoire of TCR genes, we used Satb1contional knockout micwhich the Satb1 gene was deleted in long term hematopoietic stem cells. We applied 5RACE (rapid amplification of cDNA ends) PCR technique to generate deep sequencing libraries of Tcrd, Tcrg, Tcrb, and Tcra cDNA by using a single set of primers for each TCR gene. We obtained millions of TCR sequences and unraveled the complexity of T-cell diversity. Our experimental evidence demonstrates that Satb1 has substantial influences on the TCR repertoire. SOURCE: Hao bingtao (haobingtao@gmail.com) - Southern Medical University

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