PLX124574

GSE152032: Ablation of S1PR4 triggers CD8+ T cell expansion to reduce tumor growth and improve therapy

• Organsim mouse
• Type RNASEQ
• Target gene
• Project ARCHS4

We studied the role of S1PR4 on tumor progression using the PyMT mammary carcinoma mouse model and the AOM/DSS model for colitis-associated cancer. To gain insights of the underlying mechanism how S1PR4 ablation resulted in enhanced intratumoral CD8+ T cell abundance, we performed whole transcriptome profiling of total WT and S1PR4 KO colons subjected to the AOM/DSS model (day 84) as well as FACS-sorted CD8+ T cells from WT and S1PR4 KO tumors of PyMT mice. Next-generation mRNA sequencing, in triplicates, on a NextSeq 500 (PyMT CD8+ T cells) or a HiSeq 2000 (AOM/DSS model) high-throughput sequencer was used. SOURCE: Andreas Weigert (weigert@biochem.uni-frankfurt.de) - Institute of Biochemistry I Goethe-University