PLX172940

GSE148372: RNA-Seq of brain metastatic breast cancer cell lines after knockout of lipid metabolism genes using CRISPR/Cas9 gene editing

• Organsim human
• Type RNASEQ
• Target gene
• Project ARCHS4

Brain metastasis is one of the lethal causes of breast cancer. Here we identified an altered lipid metabolism state to be associated with brain metastatic potential of breast cancer cell lines. To investigate the mechanism, we knocked out genes mediating this altered state and examined transcriptomic changes. The results revealed that these genes confer cells a state with increased lipid and cholesterol biosynthesis and that resembles the lipid metabolite state seen in the brain. These findings revealed an unappreciated machinery that cancer cells adopt for their increased adaptability to the brain microenvironment. SOURCE: Xin Jin (xjin@broadinstitute.org) - Broad Institute of MIT and Harvard