PLX210106

GSE144043: AMPK1/2 isoform-specific gene expression profile in human induced pluripotent stem cells

• Organsim human
• Type RNASEQ
• Target gene
• Project ARCHS4

AMP-activated protein kinase (AMPK) is a key regulator of energy metabolism that, upon activation, phosphorylates a wide range of substrate proteins in order to restore cellular energy balance. AMPK is a heterotrimeric protein that is composed of three subunits: , and . The and subunits are encoded by two, the subunit by three genes that are expressed in a tissue- and species-specific manner. However, neither the mechanisms of how different trimer combinations affect AMPK downstream effects nor the functional properties of single isoforms are completely understood so far. We provide evidence that AMPK1- and 2-containing AMPK trimers lead to different gene expression profiles in a model of human induced pluripotent stem cells and thereby differentially affect lineage decision.; ; The analysis of hiPSCs harboring a gene knockout for AMPK 1 (PRKAB1-/-) and for AMPK 2 (PRKAB2-/-) compared to WT hiPSCs revealed major differences in the gene expression profile in all the cell lines. This suggests an isoform-specific regulation of gene expression. SOURCE: Daniel Margerie Sanofi-Aventis Deutschland GmbH