PLX153759

GSE143373: RNAseq analysis of a genome-wide loss-of-function CRISPR/Cas9 library in haploid human embryonic stem cells (hESCs) differentiated into three germ layers

• Organsim human
• Type RNASEQ
• Target gene
• Project ARCHS4

Pluripotentstemcells can differentiate into all embryonic germ layers, a process that is orchestrated by the dissolution of pluripotency network and activation of pro-differentiation pathways. Yet, the genes essential for thesecellfate transitions in human are still elusive. Here, we used a genome-wide loss-of-function library established in haploid hPSCs and differentiated these cells into into the three germ layers. We analyzed the transcriptome of the differentiated mutant library cells to assess their differentiation status, as well as to determine lineage-specific expression of essential genes for the differentiation of hESCs into each germ layer. SOURCE: Carmel Braverman-Gross (carmel.braverman@mail.huji.ac.il) - Nissim Benvenisty Hebrew University of Jerusalem