Key Features
Enhance your research with our curated data sets and powerful platform features. Pluto Bio makes it simple to find and use the data you need.
Learn MoreKeratinocytes respond to environmental signals by eliciting induction of genes that preserve skins integrity. Here we show that the transcriptional response to stress signaling is supported by short-lived epigenetic changes. Comparison of chromatin accessibility and transcriptional changes induced by barrier disruption or by loss of the nucleosome remodeler Mi-2 identified their striking convergence in mouse and human keratinocytes. Mi-2 directly repressed genes induced by barrier disruption by restricting AP1-enriched promoter-distal sites, occupied by Mi-2 and JUNB at steady state and by c-JUN after Mi-2 depletion or stress signaling. Barrier disruption led to a modest reduction in Mi-2 expression and a further selective reduction of Mi-2 localization at stress response genes possibly through competition with activated c-JUN. Consistent with a repressive role at stress response genes, genetic ablation of Mi-2 did not prevent re-establishment of barrier integrity but was required for return to homeostasis. Thus a competition between Mi-2 repressive and activating AP1 complexes may permit rapid transcriptional response to and resolution from stress signaling. SOURCE: Katia Georgopoulos (katia.georgopoulos@cbrc2.mgh.harvard.edu) - Georgopoulos Harvard Medical School
View on GEOView in PlutoEnhance your research with our curated data sets and powerful platform features. Pluto Bio makes it simple to find and use the data you need.
Learn MoreUse Pluto's intuitive interface to analyze and visualize data for this experiment. Pluto's platform is equipped with an API & SDKs, making it easy to integrate into your internal bioinformatics processes.
Read about post-pipeline analysisView quality control data and experiment metadata for this experiment.
Request imports from GEO or TCGA directly within Pluto Bio.
Chat with our Scientific Insights team