PLX046012

GSE138170: Profiling of the reprogramome and quantification of fibroblast reprogramming to pluripotency

• Organsim human
• Type RNASEQ
• Target gene
• Project ARCHS4

Recognized as being intensive and extensive, the degree of pluripotency reprogramming, however, has not been quantitatively determined before. Here we report a novel concept, reprogramome. Fine mapping of reprogramome indicates that the amount of reprogramming genes is equivalent to 57% of the fibroblast transcriptome, with a large erasome and activatome.; We further developed a simple mathematical model to quantitate reprogramming. The total reprogramming is 26,032 LFC units (log2-transformed fold changes). Such quantification indicates that reprogramming in WNT pathways represents 2% of the total reprogramming; and reprogramming in genes involved in cellular morphogenesis represents 7.7% of the total reprogramming.; In summary, we have developed a novel concept, reprogramome, for fine profiling of the reprogramming, as well as a mathematical model for accurate quantification of the reprogramming. SOURCE: Kejin Hu (kejinhu@uab.edu) - Hu Lab University of Alabama at Birmingham