PLX070132

GSE130910: To investigate whether recover DNMT3A expression permitted adult beta cell of RapKO establishing proper transcriptome

Organsim mouse
Type RNASEQ
Target gene
Project ARCHS4

Murine islets were isolated from 8-week-old WT and RapKO mice and initially recovered in 1640 RPMI supplemented with 10% serum, 11.1mM glucose overnight. Then islets were transfected with Lenti-GFP or Lenti-Dnmt3a for 24 hours, following cultured in 1640 RPMI medium for 48 hours.Islets were harvested and stored in -80C for preparation for RNA sequencing. SOURCE: Qicheng Ni (niqicheng8@qq.com) - Rui-Jin Hospital, Shanghai Jiao Tong University School of Medicine

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