PLX218388

GSE130117: Simultaneous measurement of DNA repair activities and mRNA abundance in single cells

• Organsim human
• Type RNASEQ
• Target gene
• Project ARCHS4

New methods that measure the static abundance of DNA, RNA, and proteins in single cells are rapidly transforming biology. But these methods do not provide insight into dynamic cellular activities, precluding a complete understanding of cellular heterogeneity. We developed a method to measure enzymatic activities in single cells and used it to simultaneously measure DNA repair enzyme activities and mRNA expression. By including polyadenylated DNA hairpin substrates with defined lesions in a single-cell mRNA sequencing experiment, we measured repair activities by capturing repair intermediates and products catalyzed by activities in single cells. SOURCE: Jay,R.,Hesselberth (jay.hesselberth@gmail.com) - Jay Hesselberth University of Colorado School of Medicine