PLX275696

GSE129024: Engram-specific transcriptome profiling of contextual memory consolidation

• Organsim mouse
• Type RNASEQ
• Target gene
• Project ARCHS4

Sparse populations of neurons in the dentate gyrus (DG) of the hippocampus are causally implicated in the encoding of contextual fear memories. However, engram-specific molecular mechanisms underlying memory consolidation remain largely unknown. Here we perform unbiased RNA sequencing of DG engram neurons 24h after contextual fear conditioning to identify transcriptome changes specific to memory consolidation. DG engram neurons exhibit a highly distinct pattern of gene expression, in which CREB-dependent transcription features prominently (P=6.2x10-13), includingAtf3(P=2.4x10-41),Penk(P=1.3x10-15), andKcnq3(P=3.1x10-12). Moreover, we validate the functional relevance of the RNAseq findings by establishing the causal requirement of intact CREB function specifically within the DG engram during memory consolidation, and identify a novel group of CREB target genes involved in the encoding of long-term memory. SOURCE: Steven Kushner (s.kushner@erasmusmc.nl) - Erasmus MC