PLX066469

GSE124558: Differential roles of human PUS10 in miRNA processing and tRNA pseudouridylation

• Organsim human
• Type RNASEQ
• Target gene
• Project ARCHS4

Pseudouridine synthases (PUSs) are responsible for the installation of pseudouridine () modification in RNA. However, the activity and function of the PUS enzymes remain largely unexplored. Here we focus on human PUS10 and find that it co-expresses with the microprocessor (DROSHADGCR8 complex). Depletion of PUS10 results in a marked reduction of the expression level of a large number of mature miRNAs and concomitant accumulation of unprocessed primary microRNAs (pri-miRNAs) in multiple human cells. Mechanistically, PUS10 directly binds to pri-miRNAs and interacts with the microprocessor to promote miRNA biogenesis. Unexpectedly, this process is independent of the catalytic activity of PUS10. Additionally, we develop a sequencing method to profile in the tRNAome and report PUS10-dependent sites in tRNA. Collectively, our findings reveal differential functions of PUS10 in nuclear miRNA processing and in cytoplasmic tRNA pseudouridylation. SOURCE: Jinghui Song (sophiaatzz@163.com) - Peking university