PLX018341

GSE124410: LPS time-course in WT and Stk40 KO BMDMs

• Organsim mouse
• Type RNASEQ
• Target gene
• Project ARCHS4

Bone marrow was harvested from Rosa26CreER; Stk40+/+ (WT; n = 3) and Rosa26CreER; Stk40loxp/loxp (Stk40 KO; n = 3) mice and differentiated for 6 days in the presence of 100 nM 4-OHT to generate WT and Stk40 KO bone-marrow derived macrophages (BMDMs).; 2. On day 7 following differentiation BMDMs were treated with 100 ng x ml-1 LPS and harvested at 0 hrs, 6 hrs, 16 hrs, and 32 hrs following LPS exposure.; 3. The cells were snap-frozen at the time of harvest. RNA was extracted using the Qiagen RNeasy mini kit as per manufacturers protocol including the on-column DNase digestion.; Groups: There are cells from 3 mice x 2 genotypes x 4 time points; G1: WT 0 hr LPS; G2: WT 6 hr LPS; G3: WT 16 hr LPS; G4: WT 32 hr LPS; G5: Stk40 KO 0 hr LPS; G6: Stk40 KO 6 hr LPS; G7: Stk40 KO 16 hr LPS; G8: Stk40 KO 32 hr LPS SOURCE: Rohit Reja (rejar@gene.com) - Genentech