PLX144674

GSE123100: Transcriptomic profile alternation of human trabecular meshwork cells under substrate stiffness stimulation

• Organsim human
• Type RNASEQ
• Target gene
• Project ARCHS4

To investigate the transcriptomic profiling of human trabecular meshwork cells by increasing substrate stiffness and construct a cell model in vitro for the molecular mechanism exploration of glaucoma, normal HTM cells were cultured on collagen-coated polyacrylamide gels of tunable stiffness with Youngs moduli ranging from 1.1 kPa to 50 kPa. Then next-generation RNA sequencing (RNA-seq) was performed to obtain the transcriptomic profile of HTMCs. The expression of selected differentially expressed genes was validated by quantitative polymerase chain reaction (qPCR). Human normal and glaucomatous TM tissues were also obtained to perform RNA-seq analysis. After substrate stiffness treatment, genes related to glaucoma, including DCN, SPARC and CTGF, were significantly increased. RNA-seq analysis also confirmed the global alternation of gene transcriptional alternation. Cells under different densities exhibited similar profile change. We found ECM related genes were greatly activated by substrate stiffness, which was consistent with the molecular alternation of glaucoma. RNA-seq data from clinical samples also support the profiling alternation of cell model.The in vitro cell model could efficiently mimic the pathogenetic process of TM cells for glaucoma patients. SOURCE: Dong Chen ABLife, Inc.