PLX125353

GSE119901: The acute myeloid leukemia associated AML1-ETO fusion protein alters the transcriptome and cellular progression in a single-oncogene expressing in vitro induced pluripotent stem cell based granulocyte differentiation model

• Organsim human
• Type RNASEQ
• Target gene
• Project ARCHS4

Here we describe the development and characterization of an induced pluripotent stem cell (iPSC) system that allows in vitro differentiation towardsdifferent mature myeloid cell types such as monocytes and granulocytes. During in vitro differentiation we inducible expressed the AML1-ETO fusion protein and examined the effects of the oncoprotein on differentiation and the underlying alterations in the gene program. Our analysis revealed that AML1-ETO as a single oncogenic hit in a presumable non-mutated background blocks granulocytic differentiation and deregulates the gene program via altering the acetylome of the differentiating granulocytic cells and inducing t(8;21) AML associated leukemic characteristics. SOURCE: Joost Martens Radboud University