PLX230091

GSE115255: RNA sequence analysis of stable versus reversible EMT events and the resultant metastases

• Organsim human
• Type RNASEQ
• Target gene
• Project ARCHS4

The ability of breast cancer cells to transiently transition between epithelial and mesenchymal states is critical to complete the metastatic process. In contrast, induction of epithelial-mesenchymal transition (EMT) through the acquisition of drug persistence is a more stable event. Herein, we utilize Her2 transformed human mammary epithelial (HMLE) cells to compare a reversible model of EMT induced by TGF-beta to a stable mesenchymal phenotype induced by chronic exposure to the ErbB kinase inhibitor, lapatinib. Indeed, only a TGF-beta cells capable of returning to an epithelial phenotype resulted in long bone metastasis (BM). These four cell populations were anylzed by RNA sequencing. SOURCE: Michael,K.,Wendt (mwendt@purdue.edu) - Purdue University