PLX142601

GSE114762: Kinetics of LPS induction in wt and Cop1-ko BMDMs

• Organsim mouse
• Type RNASEQ
• Target gene
• Project ARCHS4

Bone marrow was extracted from mice that are COP1-wt Rosa26-CreERT2 or COP1-floxed Rosa26-CreERT2 BMDMs were obtained by culturing bone marrow precursors in media containing 20% of supernatant from L929 cells. At day 4 of differentiation 4-OHT was added at 1uM to induce deletion of COP1 in BMDMs derived from COP1-floxed mice. At day 7 of differentiation, BMDMs were treated with 100 ng/ml of LPS or not. BMDMs were directly harvested in lysis buffer (from Qiagen RNeasy mini kit) at different time points (0h, 2.5h, 2.5h, 4h, 6h, 9h and 13h) following LPS stimulation.; Three BMDMs preparations per group:; G1: BMDMs from COP1-wt mice (expressing the wt allele of COP1) CRE positive.; G2: BMDMs from COP1-floxed mice (expressing the floxed allele of COP1) CRE positive SOURCE: Sarah,K,Kummerfeld (sarah.kummerfeld@gmail.com) - Genentech