PLX211649

GSE114159: Next Generation Sequencing Analysis of human hematopoietic stem/progenitor cells (HSPCs) differentiated from pluripotent stem cells

  • Organsim human
  • Type RNASEQ
  • Target gene
  • Project ARCHS4

Purpose: global gene expression profiling of H1 and iPSC derived HSPCs.; Methods: SMART-seq2 amplified polyA RNA from differentiation day 8 CD31+CD34+CD43+ and CD31+CD34+CD43- cells, and day 5 CD31+CD34+ cells cultured in medium with or without insulin supplement; Results: Modulation of key cell signaling pathways lead to highly efficient HSPC differentiation from human pluripotent stem cells.; Conclusions: Highly efficient HSPC differentiation from human pluripotent stem cells in a monolayer, chemically defined culture system. SOURCE: Jie Na (jie.na@tsinghua.edu.cn) - Jie Na Tsinghua University

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