PLX104474

GSE88716: Cell-type-specific Brain Methylomes Profiled via Ultralow-input Microfluidics

• Organsim mouse
• Type RNASEQ
• Target gene
• Project ARCHS4

Methylomic studies require substantial amounts of DNA samples and this restriction hinders applications involving scarce animal or patient samples with direct biomedical relevance. Here we report a microfluidics-based reduced representative bisulfite sequencing protocol, MIcrofluidic Diffusion-based RRBS (MID-RRBS), that permits methylomic profiling with sub-1 ng starting DNA. Using this technology, we studied DNA methylation in NeuN+ and NeuN- fractions isolated from mouse cerebellum, revealing cell-type specific methylomic patterns. We also studied the DNA methylation in NeuN+ nuclei isolated from clozapine or vehicle treated mouse frontal cortex. SOURCE: Chang Lu (changlu@vt.edu) - Chang Lu Virginia Tech