PLX142205

GSE72761: RNAseq analysis of the duodenum of intestine-specific adult Nkx2.2 mutant mice

• Organsim mouse
• Type RNASEQ
• Target gene
• Project ARCHS4

Aim: Transcriptional analysis of the duodenum of adult Nkx2.2flox/flox;Villin-Cre (Nkx2.2int) mice versus control; Methods: 2 cm of the duodenum (as measured from the stomach) of 6 week old control and mutant mice were dissected and total RNA extracted. Libraries were prepared from total RNA (RIN>8) with the TruSeq RNA prep kit (Illumina) and sequenced using the HiSeq2000 (Illumina) instrument. More than 20 million reads were mapped to the mouse genome (UCSC/mm9) using Tophat (version 2.0.4) with 4 mismatches and 10 maximum multiple hits. Significantly differentially expressed genes were calculated using DEseq.; Results: 184 genes with a p-value <0.05 were down-regulated and 211 were up-regulated. Among the down-regulated genes are most enteroendocrine hormones.; Conclusion: Nkx2.2 regulates enteroendocrine cell specification SOURCE: Lori Sussel (lgs2@columbia.edu) - Columbia University