PLX268245

GSE146526: Microglial transcriptome of Maternal Immune Activation model at different developmental Stages and after microglia repopulation

• Organsim mouse
• Type RNASEQ
• Target gene
• Project ARCHS4

To determine the transcriptomic changes underlying the MIA microglia phenotype and their reversal by PLX, we performed RNA-sequencing of magnetic CD11B beads-isolated microglia from the whole brain in Saline and MIA male and female offspring at E17, P7, P20, and P60, as well as from MG-REP male and female offspring at P60. Microglial transcriptome reveals novel MIA and repopulation modules and overlap of MIA microglial genes with ASD gene network. 14,225 microglial genes from RNA-seq data revealed distinct transcriptional signatures of immature microglia (IM module, 4681 transcripts, enriched in E17 and P7 Saline), MIA immature microglia (MIA-IM module, 2816 transcripts, enriched in E17 and P7 MIA), Juvenile microglia (JM module, 2318 transcripts, enriched in P20 Saline and MIA), Adult microglia (AM module, 3276 transcripts, enriched in P60 Saline+CTRL, P60 MIA+CTRL and P60 MIA+MG-REP), and repopulated adult microglia (REP-AM module, 1,134 transcripts, enriched in P60 Saline+MG-REP) SOURCE: Tsuneya Ikezu (tikezu@bu.edu) - Laboratory of Molecular NeuroTherapeutics Boston University School of Medicine