PLX106015

GSE140898: Direct reprogramming of fibroblasts identifies signatures of vascular dysfunction in physiological aging and Hutchinson-Gilford Progeria Syndrome

• Organsim human
• Type RNASEQ
• Target gene
• Project ARCHS4

Our goal was to identify gene expression and functional differences between directly reprogrammed vascular cells derived from young and old individuals, as wells as from healthy and Hutchinson-Gilford Progeria Syndrome (HGPS) donors. We provided a full characterization of reprogrammed endothelial and smooth muscle cells by comparing their gene expression with both the original fibroblasts and primary vascular cells, showing that reprogrammed cells express key vascular cell-identity genes and contribute to the formation of in vitro 3D vascular structures. We identified and validated biomarkers of vascular dysfunction in the context of physiological and accelerated aging typical of HGPS patients, and we demonstrated their direct contribution in the modulation of vascular permeability. SOURCE: Ling Huang (lhuang@salk.edu) - The Salk Institute