PLX141949

GSE113477: Activity-dependent aberrations in gene expression and alternative splicing in a mouse model of Rett syndrome

• Organsim mouse
• Type RNASEQ
• Target gene
• Project ARCHS4

Rett syndrome (RTT) is a severe neurodevelopmental disorder that is caused by mutations in the gene methyl-CpG-binding-protein-2 (MECP2). However, the molecular mechanism by which these mutations mediate the RTT neuropathology remains enigmatic. In this study, we stimulated MeCP2-null cortical neurons (in vitro) and brains (in vivo) of a RTT mouse model to explore the effect of the loss of MeCP2 function on the activity-dependent transcriptomes of the cortex and hippocampus, respectively, using RNA-seq. These analyses revealed that the loss of MeCP2 results in aberrant global pattern of gene expression, characterized predominantly by higher levels of expression of activity-dependent genes, and anomalous alternative splicing events, specifically in response to neuronal activity. SOURCE: Nurit Ballas (nurit.ballas@stonybrook.edu) - Stony Brook University