PLX135395

GSE113165: Age-dependent skeletal muscle transcriptome response to bed rest-induced atrophy

• Organsim human
• Type RNASEQ
• Target gene
• Project ARCHS4

Short-term bed rest is used to simulate muscle disuse in humans. In our previous reports, we found that 5d of bed rest induced a ~4% loss of skeletal muscle mass in OLD (60-79 y) but not YOUNG (18-28 y) subjects. Identifying muscle transcriptional events in response to bed rest and age-related differences will help identify therapeutic targets to offset muscle loss in vulnerable older adult populations. Purpose: To compare the gene transcriptome response between young and older adult skeletal muscle after bed rest and identify a transcriptional program that underlies rapid loss of muscle mass. Methods: RNA was isolated and sequenced (HiSeq, Illumina; DESeq, R) from muscle biopsies obtained from the vastus lateralis of YOUNG (N=9; 22.9 1.1 y, 171.6 2.0 cm, 65.6 4.6 kg) and OLD (N=18; 67.6 1.3 y, 173.7 1.8 cm, 75.7 2.2 kg) men and women before and after five days of bed rest. Results: 551 genes responded similarly and 61 genes were differentially regulated between YOUNG and OLD after bed rest. Out of 61 differentially regulated genes, 51 were altered in YOUNG (42 increased, 9 decreased) to resemble expression levels of OLD baseline values. These genes were unresponsive to bed rest in OLD. Conclusion: Our results indicate that pathways related to cell adhesion and mechanosensing were downregulated more so in OLD than YOUNG (by IPA z-score), A fibrosis and inflammation pathway was altered only in older muscle, and a subset of 51 chronically altered genes in the OLD may describe predisposition to muscle loss. SOURCE: Micah,J,DrummondDrummond Lab The University of Utah