PLX084611

GSE109684: Transcriptional activation by E2F4 independent of the RB family

• Organsim mouse
• Type RNASEQ
• Target gene
• Project ARCHS4

E2F transcription factors are central regulators of cell cycle progression and cell fate decisions in mammalian cells. Within the E2F family, E2F4 often represents the predominant E2F activity in cells. E2F4 is a transcriptional repressor implicated in cell cycle arrest and whose repressive activity depends on its interaction with members of the RB family. E2F4 often represents the predominant E2F activity in cells. Here we show that E2F4 is important for the growth of mouse embryonic stem cells. In these cells, E2F4 acts in part as a transcriptional activator that promotes the expression of cell cycle genes. Importantly, this role for E2F4 is completely independent of the RB family. Accordingly, an unbiased analysis of the E2F4 interactome shows that E2F4 functionally interacts with chromatin regulators associated with gene activation in in RB family- mutant cells. Taken together, our findings uncover a non-canonical role for E2F4 that reveal novel insights into the biology of rapidly dividing cell types. SOURCE: Julia Arand (juliaarand@googlemail.com) - Stanford University