PLX172614

GSE97655: Single-cell transcriptomics of East-Asian pancreatic islets cells

• Organsim human
• Type RNASEQ
• Target gene
• Project ARCHS4

Single-cell RNA-seq (scRNA-seq) of pancreatic islets have reported on - and -cell gene expression in mice and subjects of predominantly European ancestry. We aimed to assess these findings in East-Asian islet-cells. 448 islet-cells were captured from three East-Asian non-diabetic subjects for scRNA-seq. Hierarchical clustering using pancreatic cell lineage genes was used to assign cells into cell-types. Differentially expressed transcripts between - and -cells were detected using ANOVA and in silico replications of mouse and human islet cell genes were performed. We identified 118 , 105 , 6 endocrine cells and 47 exocrine cells. Besides INS and GCG, 26 genes showed differential expression between - and -cells. 10 genes showed concordant expression as reported in rodents, while FAM46A was significantly discordant. Comparing our East-Asian data with data from primarily European subjects, we replicated several genes implicated in nuclear receptor activations, acute phase response pathway, glutaryl-CoA/tryptophan degradations and EIF2/AMPK/mTOR signaling. Additionally, we identified protein ubiquitination to be associated among East-Asian -cells. We report on East-Asian - and -cell gene signatures and substantiate several genes/pathways. We identify expression signatures in East-Asian -cells that perhaps reflects increased susceptibility to cell-death and warrants future validations to fully appreciate their role in East-Asian diabetes pathogenesis. SOURCE: Rajkumar Dorajoo (dorajoor@gis.a-star.edu.sg) - Genome Institute of Singapore