PLX309130

GSE93657: A recessive p.Cys120Arg ENPP1 Mutation Causes a Dyschromatosis Universalis Hereditaria by Altering Melanogenesis

• Organsim human
• Type RNASEQ
• Target gene
• Project ARCHS4

Propose: We used next-generation RNA sequencing (RNA-seq) to characterize the transcriptional changes in primary human melanocytes during dyschromatosis universalis hereditaria (DUH) disease. DUH patient carried missense mutation in the ENPP1 gene (c.358T>C; p.C120R). RNA-seq was performed using mRNA extracted from primary hypo- and hyper-pigmented melanocytes isolated from CUH affected patient and melanocytes from his healthy heterozygous sibling and an aged- and ethnicity-matched control.; Results: A pairwise fold-change comparison was performed and genes were computationally filtered using a cutoff of more than 2 fold change and P<0.01. We first compared hyper-pigmented melanocytes to each control individually and then overlapped the results to obtain a list of 1041 up-regulated and 692 down-regulated genes. The same analysis was done for hypo-pigmented melanocytes to found that 535 genes were up-regulated and 520 were down-regulated. Finally, to obtain a profile of the overall differential gene expression, down-regulated genes in hyper and hypo-pigmented cells were overlapped to identify 143 genes that were down-regulated in patient melanocytes compared to controls regardless of pigmentation status. Similar analysis was performed to obtain the list of 172 up-regulated genes. We selected 36 deregulated genes, most of which were associated with melanocyte development and pigmentation signaling pathways, and validated 32 of them by Q-PCR, indicating that our RNA-Seq data was accurate and reliable.; Conclusion: Our study represents the first analysis of hypo- and hyper-pigmented primary melanocytes isolated from DUH-patient versus healthy controls in DUH pathology. SOURCE: Bruno Reversade (bruno@reversade.com) - Laboratory of Human Genetics and Embryology Institute of Medical Biology/A*STAR, Singapore