PLX168559

GSE85088: CTCF modulates Estrogen Receptor function through specific chromatin and nuclear matrix interactions [RNA-seq]

• Organsim human
• Type RNASEQ
• Target gene
• Project ARCHS4

In our work we used high-throughput sequencing methods to get insight in the role of CTCF in ER-mediated gene regulation in luminal breast cancer cells. After assessing genome-wide binding of CTCF, ER, FOXA1 and Lamin B in MCF7 cells treated with estrogen for different time points, we could correlate the interaction to the chromatin with estrogen-induced de novo transcription (from GRO-seq data) and loop formation (from ChIA-PET, Fullwood et al., 2009). We observed that CTCF binding correlates with ER-mediated transcription and its depletion can affect ER-ER loop formation and subsequently gene expression. SOURCE: Elisa Fiorito (elisa.fiorito@ncmm.uio.no) - Breast Cancer group University of Oslo (UiO)