PLX095635

GSE83781: Propargite, an environmental chemical, interactswith GWAS identified diabetes genes to impact human pancreatic -cell death [PTPN2 knockout]

• Organsim human
• Type RNASEQ
• Target gene
• Project ARCHS4

This paper describes the first time a high-content environmental chemicals screen using pancreatic -like cells derived from human pluripotent stem cells (hPSCs), and discovered that a commonly used pesticide, propargite, induces pancreatic -cell DNA damage and necrosis. More interestingly, we found out the genetic background of -like cells affects their response to propargite-induced toxicity, based on isogenic hPSC platform, including for variants GWAS identified associated with T1D, since isogenic GSTT1-/- and PTPN2-/- pancreatic -like cells are hypersensitive to propargite-induced -cell death both in vitro and in vivo. In summary, our study identified an environmental chemical that contributes to the loss of -cells and provides an innovative platform for using hPSC-derived cells to explore gene-environment interactions that impact diabetes disease progression. SOURCE: Shuibing Chen (shuibing.chen@gmail.com) - Weill Cornell Medical College