PLX209927

GSE73565: Complementary Post Transcriptional Regulatory Information is Detected by PUNCH-P and Ribosome Profiling

• Organsim human
• Type RNASEQ
• Target gene
• Project ARCHS4

Two novel approaches were recently suggested for genome-wide identification of protein aspects synthesized at a given time. Ribo-Seq is based on sequencing all the ribosome protected mRNA fragments in a cell, while PUNCH-P is based on mass-spectrometric analysis of only newly synthesized proteins.; Here we describe the first Ribo-Seq/PUNCH-P comparison via the analysis of mammalian cells during the cell-cycle for detecting relevant differentially expressed genes between G1 and M phase. Our analyses suggest that the two approaches significantly overlap with each other. However, we demonstrate that there are biologically meaningful proteins/genes that can be detected to be post-transcriptionally regulated during the mammalian cell cycle only by each of the approaches, or their consolidation. Such gene sets are enriched with proteins known to be related to intra-cellular signalling pathways such as central cell cycle processes, central gene expression regulation processes, processes related to chromosome segregation, DNA damage, and replication, that are post-transcriptionally regulated during the mammalian cell cycle. Moreover, we show that combining the approaches better predicts steady state changes in protein abundance.; The results reported here support the conjecture that for gaining a full post-transcriptional regulation picture one should integrate the two approaches. SOURCE: Tamir Tuller (tamirtul@post.tau.ac.il) - Computational, Systems, and Synthetic Biology Tel Aviv University