PLX020414

GSE72483: ROR- drives androgen-receptor expression and represents a therapeutic target in castration-resistant prostate cancer

• Organsim human
• Type RNASEQ
• Target gene
• Project ARCHS4

The androgen receptor (AR) is overexpressed and hyperactivated in human castration-resistant prostate cancer (CRPC). However, the determinants of AR overexpression in CRPC are poorly defined. Here we show that retinoic acid receptorrelated orphan receptor (ROR-) is overexpressed and amplified in metastatic CRPC tumors, and that ROR- drives AR expression in the tumors. ROR- recruits nuclear receptor coactivator 1 and 3 (NCOA1 and NCOA3, also known as SRC-1 and SRC-3) to an ARROR response element (RORE) to stimulate AR gene transcription. ROR- antagonists suppress the expression of both AR and its variant AR-V7 in prostate cancer (PCa) cell lines and tumors. ROR- antagonists also markedly diminish genome-wide AR binding, H3K27ac abundance and expression of the AR target gene network. Finally, ROR- antagonists suppressed tumor growth in multiple AR-expressing, but not AR-negative, xenograft PCa models, and they effectively sensitized CRPC tumors to enzalutamide, without overt toxicity in mice. Taken together, these results establish ROR- as a key player in CRPC by acting upstream of AR and as a potential therapeutic target for advanced PCa. SOURCE: Junjian Wang (jjwang@ucdavis.edu) - UC Davis