PLX032512

GSE52385: Next generation sequencing of the transcriptome in MCF-7 cells with/without SRA knockdown

• Organsim human
• Type RNASEQ
• Target gene
• Project ARCHS4

We employed next generation sequencing to examine whether knocking down the steroid receptor RNA activator (SRA) gene significantly affect the expression levels of certain genes in MCF-7 cells. MCF-7 cells were transfected with either a pool of four non-target control siRNAs or a pool of four SRA siRNAs for 32 hrs. 157 million reads were generated from triplicate samples of the control group; 151 million reads were generated from triplicate samples of the SRA knockdown group. Six genes were identified as significantly changed in the expression levels with the cutoff of q value 0.05, fold change 0.5 or 2, and reads per kilobase per million mapped reads (RPKM) 1. However, except for SRA itself, the other five genes were shown by real-time PCR to be only affected by one siRNA in the SRA siRNA pool. Further analysis of this dataset with different cuttoff setting may reveal true SRA-regulated genes in MCF-7. SOURCE: Linghe Xi CU Boulder