PLX009908

GSE36952: Transcriptome wide analysis of classically and alternatively activated macrophages

• Organsim human
• Type RNASEQ
• Target gene
• Project ARCHS4

Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to compare NGS-derived human M1- and M2-like macrophage profiling (RNA-seq) to microarray and quantitative reverse transcription polymerase chain reaction (qRTPCR) methods and to establish a high resolution transcriptome of human macrophages.; Total RNA was isolated from classically and aternative activated human macrophages.mRNA profiles were generated by deep sequencing og M1 and M2 macrophages from 3 donors using Illumina HiSeqSQ. The sequence reads that passed quality filters were analyzed at the transcript isoform level with two methods: Casava1.8 and TopHat followed by Cufflinks. qRTPCR validation was performed using LightCycler and SYBR Green assays. SOURCE: Andrea Nino Castro (anino@uni-bonn.de) - Genomics and Immunoregulation Life and Medical Sciences institute