PLX311154

GSE156034: RNA sequencing analysis of OTUD6B-knockdown KYSE30 cells

• Organsim human
• Type RNASEQ
• Target gene
• Project ARCHS4

Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are using RNA sequencing analysis to test the effects of OTUD6B-knockdown on the mRNA of KYSE30 cells. Methods: KYSE30 cells were transfected with negative control or siOTUD6B for 48 hours in 1640 medium plus 10% serum. Two independent replicates were plated, transfected in parallel for each negative control and siOTUD6B. Results: Log-fold changes of mRNAs between negative control and siOTUD6B group were selected with a significance threshod of p<0.05. There are 241 mRNAs were upregualted and 936 were down-regulated in siOTUD6B group comparing to negative control group. Conclusions: Our study determined the mRNA changes of OTUD6B-knockdown KYSE30 cells. SOURCE: li lei (derek16ll@hotmail.com) - Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College