PLX238150

GSE155088: Enhancer priming by H3K4 methylation safeguards germline competence [scRNA-seq]

• Organsim mouse
• Type RNASEQ
• Target gene
• Project ARCHS4

Germline specification in mammals occurs through an inductive process whereby competent cells in the post-implantation epiblast reactivate a naive pluripotency expression program and differentiate into primordial germ cells (PGC). The intrinsic factors that endow epiblast cells with the competence to respond to germline inductive signals remain largely unknown. Here we show that early germline genes that are active in the naive pluripotent state become homogeneously dismantled in germline competent epiblast cells. In contrast, the enhancers controlling the expression of major PGC genes transiently and heterogeneously acquire a primed state characterized by intermediate DNA methylation, chromatin accessibility, and H3K4me1. This primed enhancer state is lost, together with germline competence, as epiblast cells develop further. Importantly, we demonstrate that priming by H3K4me1/2 enables the robust activation of PGC enhancers and is required for germline competence and specification. Our work suggests that H3K4me1/2 is directly involved in enhancer priming and the acquisition of competence. SOURCE: Alvaro Rada-Iglesias (alvaro.rada@unican.es) - Institute of Biomedicine and Biotechnology of Cantabria (IBBTEC), CSIC/University of Cantabria