PLX258053

GSE146557: RNA sequencing of mouse CT26 knockout Pd-l1 cells rescued with WT or K262Q Pd-l1

• Organsim mouse
• Type RNASEQ
• Target gene
• Project ARCHS4

Although membrane-anchored Pd-l1 has been well-studied for its engagement with PD-1 on T cells to evade anti-tumor immunity, whether Pd-l1 regulate oncogenic signaling pathways in tumor cells remains elusive. In this experiment, to further dissect roles of the K262 residue acetylation for Pd-l1 nuclear function, we profiled RNA expression of WT or K262Q mutant mouse Pd-l1 re-expressed in CT26 KO Pd-l1 cells.; Methods: Total RNA fromCT26/Vector, CT26/WT or CT26 Pd-l1 KO cells was purified using Qiagen RNeasy mini kit (Qiagen) according to the manufacturers instructions. Library preparation and sequenceing analysis were performed by BGI-Hong Kong Co. Ltd.; Conclusions: Our study indicates that Pd-l1 acetylation modification may affect its function in nuclear. SOURCE: Chen Chu (chen_chu@dfci.harvard.edu) - Dana-Farber Cancer Institute