PLX064800

GSE143543: STARR-seq identifies chromatin masked enhancers in pluripotent mouse embryonic stem cells [RNA-Seq]

• Organsim mouse
• Type RNASEQ
• Target gene
• Project ARCHS4

Enhancers are distal regulators of gene expression that shape cell identity and regulate cell fate transitions. Mouse embryonic stem cells (mESCs) are a typical example of cells whose pluripotent identity is maintained by a complex enhancer landscape, that is drastically altered upon differentiation. Genome-wide chromatin accessibility and histone modification assays are commonly used as a proxy for enhancer location, strength and dynamics. Here, we applied STARR-seq, a genome-wide plasmid-based assay, to measure the enhancer potential of genomic loci in a plasmid context in ground-state (2i+LIF; 2iL-ESCs) and metastable (serum+LIF; SL-ESCs) embryonic stem cells. SOURCE: Wout MegchelenbrinkStunnenberg Princess Maxima Centre for Pediatric Oncology