PLX114500

GSE125780: DART-seq: an antibody-free method for global m6A detection

• Organsim human
• Type RNASEQ
• Target gene
• Project ARCHS4

m6A is a widespread modificaiton which plays important roles in the regulation of gene expression. Methods for the global detection of m6A rely on immunoprecipitation of methylated transcripts using m6A antibodies. However, these methods are costly and require large amounts of input RNA, making them prohibitive for many experiments. Here, we describe improvements to m6A detection which enable transcriptome-wide mapping of m6A residues using low amounts of input material. These methods are a substantial improvement of current techniques which will facilitate detection of m6A in limiting cell and tissue types. SOURCE: Kate Meyer (kate.meyer@duke.edu) - Duke University School of Medicine