PLX150340

GSE100040: Human TFIIH kinase CDK7 regulates transcription-associated epigenetic modification

• Organsim human
• Type RNASEQ
• Target gene
• Project ARCHS4

CDK7 phosphorylates the RNA polymerase II (pol II) CTD and activates the P-TEFb- associated kinase, CDK9, but its regulatory roles remain obscure. Using human CDK7 analog-sensitive (CDK7as) cells, we observed reduced capping enzyme recruitment, increased pol II promoter-proximal pausing, and defective termination at gene 3'-ends upon CDK7 inhibition. We also found that CDK7 regulates chromatin modifications downstream of transcription start sites. H3K4me3 spreading was restricted at gene 5'-ends and H3K36me3 was displaced toward gene 3'-ends in CDK7as cells. Together, these results implicate a CDK7-dependent "CTD code" that regulates epigenetic marks in addition to RNA processing and pol II pausing. SOURCE: David,L.,Bentley (david.bentley@ucdenver.edu) - U. Colorado School of Medicine