Example

What can I do with this analysis?

• Functional annotation of genomic regions: You'll be able to annotate your peak regions, categorizing them as promoters, enhancers, or other regulatory elements.

Suggested applications

• Disease association: Annotate peak regions between healthy and disease samples to classify regulatory elements associated with disease states.
• Regulatory element identification: Use functional annotation analysis to categorize consensus peak regions identified across multiple cell lines.
• Molecular basis of disease: Annotate differentially bound/accessible regions identified between two groups, such as treated and untreated samples, to characterize regulatory elements associated with specific experimental conditions.

Plot types & customizations

• Stacked bar plot: A special kind of bar plot, stacked bar plots show functional annotations attributed to each peak set. Each bar represents a peak set, and segments within the bar represent frequencies of different annotations. You'll be able to customize properties such as:

  • Transposing the x- and y-axis
  • Annotation colors

• Pie chart: Visualize an individual peak set using a pie chart, where each slice represents a distinct annotation and its size corresponds to the annotation's frequency. You'll be able to customize properties such as:

  • Selecting a peak set to visualize
  • Showing percentages on the chart
  • Annotation colors

Additional resources

• Functional Annotation Analysis help doc