Analyze gene expression at a genome-wide scale to uncover differential expression, altered regulatory pathways, transcriptional networks, and individual biomarkers and targets. Bulk RNA sequencing (bulk RNA-seq) offers a powerful approach to analyzing the transcriptome of complex samples, providing insights into the gene expression profiles of whole tissues or cell populations. This technique is invaluable for understanding the biological context of various states, such as disease vs. healthy conditions, and identifying key genes and pathways involved in specific processes. Pluto Bio enhances bulk RNA-seq data analysis with scalable, efficient, and user-friendly tools, supporting researchers in their quest for comprehensive biological understanding.

Pluto provides a comprehensive solution for bulk RNA-seq data analysis. Our platform supports the entire workflow from data preprocessing to differential gene expression analysis, making it easier for researchers to uncover biological insights from complex datasets. By simplifying the analysis process, Pluto enables scientists to focus more on their research questions and less on the technicalities of data analysis.
With our intuitive platform, gain deep insights into the gene expression profiles of your samples. Pluto Bio's tools facilitate the exploration of gene expression across different conditions, treatments, or time points, helping to advance your research in fields such as oncology, immunology, and developmental biology.
Pluto democratizes access to advanced bioinformatics tools, allowing researchers with varied expertise levels to perform bulk RNA-seq analysis. Whether you are looking to identify biomarkers, study disease mechanisms, or explore the effects of drug treatments, our platform equips you with the necessary tools to achieve your research goals.


Single-cell data is transforming translational research. From oncology to immunology, the ability to resolve cell populations at unprecedented resolution is fueling breakthroughs in target discovery, biomarker identification, and patient stratification.
Compare top bioinformatics platforms—Pluto Bio, Partek Flow, Basepair, and Rosalind—to find the best fit for your NGS data analysis needs. Assess supported assays, analyses, visualizations, and collaboration features.
Discover the differences between RNA-seq and scRNA-seq, their applications in target discovery, and how pseudobulking bridges bulk and single-cell analysis. Unlock insights into gene expression for drug development and personalized medicine.
Discover top alternatives to Rosalind for bioinformatics and computational biology. Explore platforms like Pluto Bio, BaseSpace, GenePattern, and more to find the right solution for your research needs.
Learn the basics of RNA-seq, including its methodology, applications, experimental design, and data analysis techniques. This 101 overview is perfect for biologists and bioinformaticists investigating transcriptomics for the first time.
Ready to experience the most intuitive scRNA-seq experience yet?
Dive into the world of Precision Run-On sequencing (PRO-seq) in this comprehensive blog post. Discover the origins of PRO-seq, how it compares with traditional RNA-seq, and explore a step-by-step guide on conducting a PRO-seq experiment. Uncover how this innovative technology is advancing our understanding of transcriptional dynamics and revolutionizing genomic research.
RNA sequencing (RNA-Seq) has revolutionized the way we study gene expression. The data deluge it produces, however, presents a critical question: how can we make valid comparisons between different samples or conditions?
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We’re proud to highlight a new paper citing Pluto, published today in Cells by Dr. Holger Russ’ lab at the Barbara Davis Center for Diabetes, University of Colorado School of Medicine.