Next-generation sequencing (NGS) is a powerful tool used to generate high-throughput sequencing data. It has revolutionized the field of genomics and has numerous applications in areas such as functional genomics, gene expression analysis, and variant discovery.
NGS generates large amounts of data that require specialized software for analysis. There are a wide variety of NGS software tools available, each with its own strengths and capabilities. Some examples of NGS software include:
Read alignment tools: These tools align the NGS reads to a reference genome, allowing researchers to identify the genomic location of each read. Examples include Bowtie ,BWA, STAR, and TopHat2.
Variant calling tools: These tools identify variations in the genome, such as single nucleotide polymorphisms (SNPs) and indels. Examples include DeepVariant, Platypus, Mutect2 and VarScan.
Gene expression analysis tools: These tools quantify the expression of genes in different samples and can be used to identify differentially expressed genes. Examples include edgeR and DESeq2.
Overall, NGS software is an essential component of modern genomics research. The right software can greatly enhance the efficiency and accuracy of NGS data analysis and allow researchers to fully leverage the power of this technology.
Contact us to learn more and to get a better understanding on how the Pluto platform uses these tools to help our clients.